Coding
Part:BBa_K2933132:Design
Designed by: Wenhui Gong Group: iGEM19_TJUSLS_China (2019-09-15)
His+Linker a+Sumo+Linker b+SHD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 256
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 256
Illegal NheI site found at 33 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 256
Illegal BglII site found at 145
Illegal BamHI site found at 344 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 256
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 256
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized in E. coli BL21(DE3).
Source
NCBI